Molecular analysis of sialoside binding to sialoadhesin by NMR and site-directed mutagenesis
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چکیده
منابع مشابه
Molecular analysis of sialoside binding to sialoadhesin by NMR and site-directed mutagenesis.
The molecular interactions between sialoadhesin and sialylated ligands have been investigated by using proton NMR. Addition of ligands to the 12 kDa N-terminal immunoglobulin-like domain of sialoadhesin result in resonance shifts in the protein spectrum that have been used to determine the affinities of sialoadhesin for several sialosides. The results indicate that alpha2, 3-sialyl-lactose and ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1999
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj3410355